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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. |
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Afiliação: |
LAUDIANE ZANELLA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JOÃO CARLOS BESPALHOK FILHO, UFPR. |
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Título: |
RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
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Conteúdo: |
From the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants. MenosFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histoch... Mostrar Tudo |
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Thesaurus Nal: |
Populus alba; Populus tremula. |
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Categoria do assunto: |
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Marc: |
LEADER 02291nam a2200181 a 4500
001 2118433
005 2020-02-04
008 2019 bl uuuu u01u1 u #d
100 1 $aZANELLA, L.
245 $aRNA interference supression of DOF transcription factor from Populus tremula x Populus alba.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 322$c2019
500 $aNa publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants.
650 $aPopulus alba
650 $aPopulus tremula
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGERHARDT, I. R.
700 1 $aBESPALHOK FILHO, J. C.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca |
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| Registros recuperados : 50 | |
| 2. |  | KAEL, D. C.; GERHARDT, I. R. Estabelecimento de uma coleção de plasmídeos para transformação genética de eucalipto. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 11., 2012, Colombo. Anais. Colombo: Embrapa Florestas, 2012. (Embrapa Florestas. Documentos, 240). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 8. | | GERHARDT, I. R.; OKURA, V. K.; DANTE, R. A.; ARRUDA, P. A gene expression atlas of Vellozia nivea, a desiccation-tolerant species from the Brazilian campos rupestres. In: INTERNATIONAL PLANT & ANIMAL GENOME, 28., 2020, San Diego. Abstracts... Livingston, NJ: Scherago International, 2020. PE1028.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 9. | | GRUNENNVALDT, R. L.; KAEL, D. C.; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M.; GERHARDT, I. R. Identificação e clonagem de promotores raiz-específicos de eucalipto. Biochemistry and Biotechnology Reports, v. 2, n. 3, p. 50-53, 2013. Edição dos anais do 3º Simpósio de Bioquímica e Biotecnologia, 2013. Londrina.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 11. | | ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 12. | | ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 322. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 13. | | FERREIRA, P.; PAES, N. S.; SOUZA, C. R. B.; GERHARDT, I. R.; VENTURA, M.; SA, M. F. G. de. Arcelin protein: antigenic relations, developing seeds study and imunocytolocalization. In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 1., 1993, Brasilia. Programa e resumos. Brasilia: EMBRAPA-CENARGEN, 1993. Poster 031. Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | LAIA, M. L. de; GERHARDT, I. R.; ABAD, J. I. M.; DEGENHARDT-GOLDBACH, J.; GONÇALVES, J. F.; MISSIAGGIA, A. A. A biotecnologia e o eucalipto do futuro. In: VALE, A. B. do; MACHADO, C. C.; PIRES, J. M. M.; VILAR, M. B.; COSTA, C. B.; NACIF, A. de P. (Ed.). Eucaliptocultura no Brasil: silvicultura, manejo e ambiência. Viçosa, MG: SIF, 2014. p. 39-67.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Florestas. |
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| 15. | | GERHARDT, I. R.; GIACHETTO, P. F.; YAMAGISHI, M. E. B.; LOBO, F. P.; TEIXEIRA, J.; PENCHEL, R. M.; MISSIAGGIA, A. Comparative transcriptome analysis of eucalyptus genotypes that differ in carbon allocation. In: INTERNATIONAL PLANT & ANIMAL GENOME, 20., 2012, San Diego. Abstract... Jersey City: Scherago International, 2012. Não paginado. 1 pôster.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Florestas. |
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| 17. | | GERHARDT, I. R.; LEITE, A.; PAES, N.; ROMANO, E.; AZEVEDO, M.; BLOCH JUNIOR, C.; GROSSI de SA, M. F. Isolation and partial characterization of a genomic clone of arcelin-5 protein. In: ENCUENTRO LATINO AMERICANO DE BIOTECNOLOGIA VEGETAL, 2., 1995, Puerto Iguazu, Argentina. REDBIO95. [S.l.: s.n.], 1995. n.D-11.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | SANTOS, P. E. T. dos; PALUDZYSZYN FILHO, E.; SCHUHLI, G. S. e; GERHARDT, I. R.; OLIVEIRA, V. R. de. Melhoramento genético de eucaliptos para desenvolvimento de cultivares destinadas a múltiplos usos da madeira e de populações com potencial para superar possíveis adversidades ocasionadas por mudanças climáticas. In: SOTTA, E. D.; SAMPAIO, F. G.; MARZALL, K.; SILVA, W. G. da (org.). Estratégias de adaptação às mudanças do clima dos sistemas agropecuários brasileiros. Brasília, DF: MAPA, 2021. p. 48-49.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Florestas; Embrapa Semiárido. |
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| 19. | | FERREIRA, P.; PAES, N.; GERHARDT, I. R.; YOKOYAMA, M.; SANTANA, E.; HARRIS, N.; GROSSI de SA, M. F. The distribution of arcelin in developing seed and larval gut. In: ENCUENTRO LATINO AMERICANO DE BIOTECNOLOGIA VEGETAL, 2., 1995, Puerto Iguazu. Argentina. REDBIO95. [S.l.: s.n.], 1995. n.B-11.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 50 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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